FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5451914
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG23100.020.74261
GTTCTAG23000.020.5171381
CCGTATA9000.019.32336145
GTACTAA18450.019.2810631
GTCCTAG19350.019.1346471
GTCTTAG19150.018.5762711
GTACTAT16950.017.5608441
TCTGTAC39900.017.4464513
GTGTAGG29900.016.9964451
GGTCTAA17150.016.9327351
GGTCTAG17000.016.6550871
GTGCTAG22950.016.4494691
GTGCTAA23950.016.3688981
GTCCTAA19400.016.0915551
TGTACCG11800.015.9669225
GTATAGA21550.015.8336781
GCTCTAG24300.015.535611
TACTAAG19800.015.389912
GTACAGG39650.015.3803891
GTATATG27950.015.3250331
Produced by FastQC (version 0.11.5)