FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5379542
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19800.021.2398261
GTACATA24150.019.2154671
GTACTAG12600.018.9902861
GTCCTAG18400.017.7330271
GTCCTAA18850.017.309691
GCTCTAG24000.017.220741
GTTCTAG22750.017.2107771
GTACTAA16600.017.0350991
GTGCTAG20750.016.4235841
TCTAACC20850.015.9951173
GGGGGGG3137550.015.964827145
GGTCTAG15000.015.951841
CGTCTTG30400.015.740193145
GTTCTAT23200.015.6268041
GTATAGA19300.015.0276411
GTATATG27700.014.9204951
TCTAGAC16550.014.894183
GTATATA30700.014.8795691
GTCTAGA18650.014.7738241
GCTTTAG32500.014.7247761
Produced by FastQC (version 0.11.5)