FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5379542
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA13650.025.492453145
TATACCG6900.023.1115045
GTATTAG20900.022.555051
CGCCGTT18200.021.907578145
GTCCTAG18200.021.1193791
GCCCTAG19200.019.2639641
GTCTTAG19100.018.985121
GGTCTAG15800.018.81931
TGTACCG11650.018.6659415
GTACTAG11300.018.6121371
GTTCTAG23400.018.595681
CTAGGGT14750.018.1824424
GTATTAA30300.017.9512751
GTACAGG37850.017.8194271
GTCCTAA18250.017.4850351
GCTCTAG26000.017.0150471
CTAGACT16850.016.7767244
TTATACC48100.016.729422145
GTACATA25150.016.7250211
GTGCTAG21300.016.683731
Produced by FastQC (version 0.11.5)