FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5395649
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT15950.026.35747145
CCGTATA12900.022.47536145
GTTCTAG24100.021.0639041
GTACTAG10200.019.1964741
GTGCTAG21950.018.1712991
GTATTAA30900.017.8366371
GTACAGA36800.017.5388071
CTATACT18600.017.5373574
TTATACC48800.017.378105145
GTGCTAA23000.016.7111321
GGTCTAG15700.016.6287941
GTACAGG35800.016.6107271
GTCCTAC19250.016.5760021
GTACTAA18450.016.508621
GCCCTAG20450.016.3125691
GTCCTAG17500.016.1616021
CCCTTAG21150.016.1155571
GGGGGGG2166950.015.931919145
CTGTACT37100.015.8261644
GTCCTAA19500.015.6196941
Produced by FastQC (version 0.11.5)