FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8938639
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG34100.022.754771
GTATTAG28750.022.4489081
GCGAATG115100.022.044966145
GTATAGG24500.021.9032571
CGTCTTG40950.020.890308145
GTACTAG18600.019.1040841
CGTCTTA26850.017.010342145
GCTCTAG42850.016.7543451
ATAGTAC27050.016.6213043
GTTCTAG40100.016.45661
GTACATA36150.016.4493471
GTACTAA25650.016.3977281
GGATTAG33700.016.3541051
GGGGGGG4076550.015.818668145
GGTCTAA29000.015.7538111
CTAAGCG11100.015.6793794
GTATAAG29150.015.6727451
ACTATAC27150.015.4916913
GTATATG41150.015.3317771
TGTACCG13750.015.2945235
Produced by FastQC (version 0.11.5)