FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8748626
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18400.024.4340761
GTGCTAG35700.019.9058151
GTATTAG28900.019.0694371
GTACTAA24200.018.8776231
GTCCTAG33050.018.2107871
GCTCTAG40350.018.1509741
CGTCTTG52900.017.953264145
GTCCTAA30150.017.7978081
GTATTAA42500.016.8915041
GTCTTAG33100.016.649751
GCGAATG134400.016.56025145
GTTCTAT37000.016.4626461
GGGGGGG3773050.015.944418145
GTTCTAA40750.015.6594721
GTACTAT25200.015.5387221
TACTAGA28050.015.5101382
GGTCTAG28250.015.4012111
GTGCAGG129650.015.3809181
GTATACG7553.45608E-1115.3672141
GTATAGG25550.015.3258641
Produced by FastQC (version 0.11.5)