FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8659961
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG39400.021.3456481
GTATTAG27950.019.7142091
GTACTAG15150.018.1852191
CTATACT28700.017.1785744
GTCCTAG33800.016.9456391
GTACGGA10300.016.8935761
GCGAATG139000.016.586601145
CGTCTTG57350.016.560862145
GTGCTAG32850.016.5528761
GTACTAA26700.016.2924941
GTATTAA44700.016.2195971
GTATAGG21550.016.1488481
GCTCTAG40550.016.09161
CTGTACT67750.015.9454614
GTCTAGA34150.015.7104471
TGCCGTG65650.015.6819145
GTATATG41150.015.6807831
GTACTAT25900.015.6760221
GGGGGGG3716250.015.66201145
GTACAGA62150.015.631881
Produced by FastQC (version 0.11.5)