FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8659961
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT22550.022.825993145
CCGTATA22850.022.526306145
GTACTAA25900.020.169461
GTTCTAG39050.019.8803621
GTATTAG30000.019.3477421
GTCTTAG31850.019.1351281
GTACTAG17450.019.125991
GTCCTAA31750.018.966881
GTCCTAG33100.018.4125041
GGTCTAA28500.018.3294411
GTGCTAG32350.018.1665421
GCCGACC40600.017.320625145
TTACGTT31350.017.112444145
GTTCTAA42700.016.8216611
GTGTTAG37850.016.4851971
GTACATA34900.016.4234051
TCTAGAC33250.016.1379433
GACGACA43050.015.998097145
GCCCTAG35000.015.9618871
GTACAGA65550.015.9386431
Produced by FastQC (version 0.11.5)