FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8819951
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG31500.021.4101921
CTAGATG114600.020.305834145
CTATACT31000.019.4153354
GTATATA51550.019.2725891
CACGAGC110650.018.934195145
GTGCTAG35250.018.9267851
GTATAGG23400.018.2845521
GTCTTAG31400.018.0141221
GGTCTAG28000.017.3526131
CTAGACT29800.017.2770884
GTATTAA45050.016.9021781
CCTAGAC31750.016.9011573
GTCCTAA30950.016.8701921
CTCCGAC39250.016.807463145
GTGTAGG45800.016.6253951
TAGATCG27200.016.524305145
GTTCTAA40700.016.2141861
GTATATG41600.016.2120441
GTATTAT47600.016.1490541
GCCCTAG32550.016.0409341
Produced by FastQC (version 0.11.5)