FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8819951
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT13900.027.636946145
CCGTATA13500.023.623701145
GTACTAG18150.020.0163541
GTGCTAG33000.019.375831
TTATACC65100.018.92766145
GTACTAA27200.018.69911
GCCTTAG34350.018.6143361
GTCCTAG32900.018.3304791
CTATACT33400.018.2381614
CTGCCGC154500.017.967981145
GTGCTAA36150.017.8884741
GGTCTAA26950.017.794131
GTATTAA47650.017.6883241
GTCCTAA27950.017.417451
GTATTAG31350.017.3826241
GTCCTAT28250.016.9752861
GCTCTAG41150.016.9509091
GTACTAT26200.016.9168761
GCCGTAA13750.016.868492145
GTACATA37850.016.7010961
Produced by FastQC (version 0.11.5)