FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8650907
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG23250.020.897311
GTATTAG28000.020.2011051
GCCCTAG34250.019.0555061
GTATATA48500.018.5403751
CTAGACT30150.018.5187574
GTCCTAA31300.017.607911
GTACAGG62700.017.5798281
CGTCTTA28600.017.237469145
TCTAGAC33050.017.1132143
GTGTATA36050.017.0982721
GTCTAGG31900.017.04941
GCTCTAA41150.016.741421
GGTCTAG28050.016.545721
GTGCTAG35450.016.364861
CTATACT29850.016.2756714
GGGGGGG3795750.016.112747145
GTATTAA41550.016.0566651
GTACATA37650.015.9864371
GTTCTAA42550.015.8497321
GTATATG43100.015.8157261
Produced by FastQC (version 0.11.5)