FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8622902
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT19150.028.770952145
CCGTATA22050.022.685581145
GTCCTAG35800.021.4833811
GTACTAG16700.019.5513081
GTACTAA26500.019.4398211
CTATACT32450.019.4391354
GTTCTAG36100.019.093971
TACGCGA3051.2726019E-519.0236362
GTGCTAG34050.018.9649931
GTCCTAA30750.018.8766381
GTATTAG31600.018.8281041
GTACATA40000.018.3206631
GTTCTAA40750.018.1615261
GTCTAGG31250.018.1102471
GGTCTAA26350.017.898331
TATACCG7000.017.6078225
GTCTTAG30200.017.5386331
CCTCTAG52900.017.4191861
GTGCTAA33550.017.301241
GTGTTAG34500.017.2454511
Produced by FastQC (version 0.11.5)