FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5187950
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG12550.018.483651
GTTCTAG14650.018.4337481
GTATTAG11500.017.7627751
GTACTAG7300.016.5997621
GTATAGG9250.016.4690151
TCTATAC11950.015.0528723
GTCCTAA11600.014.9234311
GCTCTAA15100.014.445091
GTATTAA19300.014.3512371
GTACAGG22800.014.274131
GTACTAA10900.013.9759981
GTCCTAC10500.013.8489431
GTATTAT17350.013.5695711
GTGCTAG11500.013.5478791
GTATATG15900.013.2827931
GTCTAGA12400.013.1229911
GGTCTAT7750.012.9554631
GTGTTAA17150.012.9202981
GGTCTAA9400.012.8913041
GTTCTAA16450.012.8386861
Produced by FastQC (version 0.11.5)