FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5075508
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10750.017.7078741
GTATACG2603.1794116E-815.97423651
GTCTAAG10900.015.8765351
GTTCTAG13450.015.4397821
GTATATA19800.015.3825981
GTATTAA17700.015.2522361
GTCCTAC9900.014.6833891
GTCTTAG12150.013.9582831
GTACTAC8050.013.7583481
GTCTAGG13050.013.5260771
GTACTAA11500.013.2424111
GTACTAG7100.013.1618721
GTATAGG10000.013.1521221
CAGTACT18450.013.1316164
CCAGTAC18600.013.0255863
GTTCTAA15450.012.9930681
CTAGACT10700.012.9387624
GTACAAG16050.012.9386341
TATACTG15600.012.646525
GCTAGAC9050.012.6205293
Produced by FastQC (version 0.11.5)