FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6090139
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6750.019.4258961
GTATTAG11900.016.5283341
GTCTTAG13750.015.8102621
GTACTAA11750.014.97731
GGTCTAG11100.014.921741
GTTCTAA17750.014.7746251
GTATAGG10350.014.6694391
GCTAGAC9750.014.5106733
GTGCTAA15400.014.3403731
CTAGACC12200.014.142834
GTATTAA18350.014.1034841
TGTACCG6400.014.0191965
GTCTTAA15350.013.7126891
GTCCTAT13100.013.6972461
GGTCTAA11100.013.6782621
GTCTAGA14650.013.6612851
GTTCTAG16100.013.5025531
TAGATCG3102.8382237E-713.3582415
GTACATG23500.013.0684281
GTCCTAG16450.013.0054981
Produced by FastQC (version 0.11.5)