FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6090139
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG17700.016.8131451
GTATTAG13850.015.9902021
GTCCTAG15200.015.935961
GTACTAA11100.015.8990411
GTACTAG7100.015.5960761
GTGCTAG14600.015.40581
GTATACG3503.6379788E-1014.8301981
GTCCTAT13600.014.503061
GCTCTAG16350.014.1801491
GTGTTAG15400.014.1560981
GTCCTAA13550.014.0458211
GTATTAA19100.013.9502211
GTATAGG10350.013.7077851
GCCCTAG13300.013.5293041
GTGCTAA15100.013.5206881
GCTATAC9600.013.3164843
GTTCTAA17350.013.16340351
GTCTTAG12650.013.1302941
TCAGTAC17500.013.0306173
CTAGACG2952.354749E-612.8750154
Produced by FastQC (version 0.11.5)