FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5968920
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7050.018.5980431
GTACTAA12250.017.7450491
GGTCTAA11900.015.3674451
GTCTAGA13000.015.1287941
TATACCG3756.730261E-1114.7234225
GTGCTAG14350.014.6673171
GTTCTAG15300.014.6586741
GTGCTAA13750.014.5545271
GTCTTAG13050.014.54202651
GGTCTAG11250.014.4151141
GTCCTAG15200.014.301111
GTATTAG12800.014.28692151
GTATTAA17950.014.2245991
GTATAAG13000.014.0671231
GTCCTAC11950.013.8594521
GGCCTAG16850.012.9007031
GTACCGT5103.6379788E-1212.855936
TGCACTG37600.012.6651785
CTATACC13100.012.64394954
CTAATAC13400.012.6182883
Produced by FastQC (version 0.11.5)