FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5838085
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG12750.015.6904221
GTGCTAG14150.015.6005671
GTCCTAG14200.015.3027341
GTATTAA18050.015.2872591
GTCTTAA14600.014.8834821
GGTCTAA10550.014.7121741
GTCTTAG13150.014.6885571
GTACTAG6650.014.5228961
GGTCTAG12000.014.0841841
GTATAGG10150.013.9326791
GTGTTAG14500.013.7967511
TAGACAG21550.013.7653285
GTTCTAA16500.013.3786671
GTTCTAG15750.013.3587581
GTCTATA12250.012.9520511
TATACTG16000.012.9350075
GTGCAGG52850.012.8569531
GTATATA21850.012.7864621
CTAGGAC13750.012.2918623
GTCCTAA11550.012.2438691
Produced by FastQC (version 0.11.5)