FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9688941
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA68600.031.765352145
GGGGGGG642100.019.582115145
GTACTAA28650.018.0232731
CTCGTAT33350.015.771827145
TAGCACG11806.490287E-414.858487145
GTCCTAG38300.014.8303771
GTGCTAG38300.014.8303771
GTACTAG20350.014.3787641
GTTCTAA45700.014.3121361
GTCTTAG34150.014.2805191
GTATTAG31450.014.0470731
TATGCCG34150.013.935464145
GGTCTAA28450.013.914981
GTACTAC22400.013.8312521
GTATATA53450.013.7397251
CTATACT34250.013.5689014
GTCTATA31400.013.5212811
GCTCTAG42750.013.286631
GTCTAGA34150.013.2724821
GTCCTAA32500.013.2401731
Produced by FastQC (version 0.11.5)