FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9681935
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA67500.040.410343145
GGGGGGG642300.020.76197145
GTACTAG17950.017.5295521
GTCCTAG39350.016.8649831
GTATTAG31550.015.9571721
GTACTAA31400.015.8512041
GTGCTAG37600.015.215451
GCTCTAG45350.014.6336721
GTATTAA45900.014.5829651
GTATGCG10700.005500182514.162503145
CCTGTCG30352.1827873E-1114.146949145
GTTCTAG44550.014.1259481
GGTCTAG30600.013.8351191
TATACTG43950.013.6685755
ATATCGT13050.001305213413.547528145
GTCCTAA32650.012.9664521
GTATATA53850.012.9612461
CTGTACT75950.012.881014
GTCTTAG35650.012.8381691
GTGCTAA37950.012.8138531
Produced by FastQC (version 0.11.5)