FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9383083
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA75350.035.070415145
GGGGGGG505350.022.25991145
GTCCTAG39400.016.053381
TATGCCG44450.015.816994145
GTGCTAG36650.015.6890281
GTCCTAA32300.015.6657121
GTATTAG31250.015.2720761
GTACTAG19750.015.1393151
ATCGTAT17954.687281E-714.856822145
GTACTAA26750.014.1869851
GTATTAA47800.013.9540451
GTACAGG66300.013.6162061
GTGCTAA35300.013.1941181
GGTCTAA27000.012.9908061
GTATAGG25250.012.9802631
TCTATAC30150.012.9691093
TCGTATG41250.012.929936145
CGAGACA31901.0186341E-1012.919805145
GTGTAGG47300.012.8858991
GTATATA52500.012.5953011
Produced by FastQC (version 0.11.5)