FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9399000
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA75700.038.997715145
GGGGGGG586300.023.006275145
GTCGCTT27450.017.487091145
GTACTAG17950.016.6425931
ATGCCGT52400.016.489258145
GTACTAA29300.015.2935771
GTGCTAG40250.015.2721441
GTATTAG30200.015.2182651
GTCCTAG35600.015.0077311
TCGTATG48150.014.953914145
ATTAGAC31850.014.6102523
GTATAGG26600.013.8223051
TATGCCG47300.013.700377145
GTCTCGT32209.094947E-1213.416726145
CTATACT34750.013.3910484
GCTCTAG41950.013.1468391
GTATTAA46850.012.9980561
GTCCTAA32000.012.9260151
GTGTATA40650.012.8606511
GTTCTAG43800.012.72271251
Produced by FastQC (version 0.11.5)