FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9399000
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA74350.027.764967145
CCTGTCG37500.023.962364145
CCGTATA9703.242876E-722.53357145
TAGATCG8959.9630786E-518.994778145
TGACGCG15604.5893103E-918.681677145
CGCGTAT6501.576991E-718.681677145
TCGCCGT36650.018.554245145
GTCCTAG36800.017.6434611
GCCGTAT28900.016.807045145
GTCTCGT32650.016.364347145
GTTCTAA44000.016.1928061
GTCTTAG32600.016.0389861
CGCCGTT11004.814198E-415.454842145
GTTCTAG43950.015.296081
GTACTAG19100.015.0414541
GTGCTAG35150.015.0388861
GTATAGG25250.014.5637011
TCGCATA78200.014.285988145
CTATACT33650.014.1661574
GTCCTAA33700.014.1514631
Produced by FastQC (version 0.11.5)