FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8875528
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA72200.033.2772145
GTATTAA64550.017.99281
GGGGGGG490500.017.41616145
GTACTAG21100.017.1122251
GTATTAG41850.016.2487771
GTCTTAG38500.015.6306471
GCTCTAG39750.015.4418981
GTTCTAG48550.015.2459171
GTACTAA34100.014.6474291
CCTATAC29250.014.6074723
GTATATA85650.014.5438941
GTACTAT34900.014.48411
GTCCTAG36700.014.42961
CTATACT43950.014.3772374
GTATTAT68150.014.0400591
GTATAAG39450.013.8813631
GTCTTAA49850.013.7618671
GTGCTAG37350.013.6951251
GGTCTAA36500.013.6843111
CCTGTCG24202.4738256E-1013.576965145
Produced by FastQC (version 0.11.5)