FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8875528
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20850.020.8293721
GTCCTAG38800.019.4324721
CTGTCTA68700.019.37277145
GTATTAG43300.017.2736261
GGTCTAG28950.017.0850031
CCTGTCG22950.016.951418145
GTATTAA64500.016.2719381
GTCCTAA35350.015.6981391
GTGCTAG37350.015.6650181
CGACGAA11801.0808681E-515.616928145
GCCGTAA10607.5712545E-515.45323145
GTGTTAG42900.014.6226411
CGACTAT9907.9023506E-414.477646145
GTATATA85450.014.4707681
GTTCTAA53000.014.4536531
GTATAGG29650.014.2404311
GTACTAA34350.014.2235511
GTTCTAG43950.013.9988071
TTATACT70350.013.61237054
GCTCTAG40350.013.6034041
Produced by FastQC (version 0.11.5)