FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8916933
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA72400.021.520542145
GTATTAG41350.020.0492531
GTATTAA66850.017.6136471
GGGGGGG435200.016.850637145
GTACTAG21500.016.765151
GTCCTAG36150.016.618291
GTACTAA34150.015.3046411
GGTCTAG27100.015.2958781
GCTCTAG41000.015.0920421
GTCTTAG38550.014.9603411
GTATAGG29400.014.9166111
GTCCTAA35850.014.9140451
TAACCCG9250.014.2886165
GTATATA86150.014.2255651
GTATATG59050.013.9378341
GTATAGA44200.013.72757151
GTTCTAA53550.013.5743961
GTACTAT35850.013.4058831
CCTGTCG20154.9594746E-813.402901145
GTATAAG38550.013.4019721
Produced by FastQC (version 0.11.5)