FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8916933
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA70050.019.174574145
GTATTAG42550.019.1120511
GTACTAG22550.017.3636051
GTACTAA35950.016.0859031
GTATTAT73400.015.4288971
GTTCTAA55100.015.4148961
GTGCTAG38150.014.8424781
GTATTAA68600.014.8400611
GGTCTAG30050.014.633611
GTCTTAG37900.014.6225031
GTCCTAG38200.014.5076671
GTCCTAA35800.014.4706661
GTTCTAG45650.014.3833081
GTATAGA43800.014.303171
GTACATA54100.014.2523271
CTAGACT35100.014.2268214
GCTCTAG40300.014.0506331
CCTGTCG22359.913492E-1013.868651145
CTATACT43450.013.8467254
GTATATA88450.013.6889871
Produced by FastQC (version 0.11.5)