FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8615239
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA75950.035.041916145
GTACTAG21400.018.864641
GGGGGGG384400.018.656734145
GTATTAG39600.018.4110151
GTATTAA65400.015.6624121
GTACTAA33450.015.3112361
GTCCTAG32850.015.22405051
GTCCTAA33300.014.6564321
CCTGTCG25051.8189894E-1214.598528145
GTATAGG26900.014.3355771
CTATACT38250.014.3353254
GTTCTAG45000.014.1932181
GTGCTAG37300.014.0539341
GTCTATG42200.013.9926871
GTATATG59800.013.9048211
TAGGACT41800.013.694464
GTATAGA43150.013.6846211
GTCTTAG36300.013.4451581
GTTCTAA49350.013.4305261
TCTAGAC35500.013.4089973
Produced by FastQC (version 0.11.5)