FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8615239
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA74900.024.382338145
TGACGCG9204.0034138E-822.303825145
CCTGTCG24850.021.469114145
CGACTAT11508.403731E-1021.411673145
GTACTAG20250.021.1259751
GTGCTAG37100.018.0273741
GTCGCGT5800.00911140317.68924145
GTATTAG40500.017.257841
GTATTAA62950.017.037571
GTCCTAG32450.015.9686121
GTTCTAA50800.015.8936821
TATACTG53750.015.4587335
GTACTAA34550.015.34680751
GTCTTAG39750.015.3097341
GTTCTAG42200.014.9920231
GGTCTAG27700.014.7915121
GTCCTAT35000.014.4608781
GTCCTAA36900.014.2061461
CTAGTCG43450.014.167677145
GCCCTAG33800.014.0829561
Produced by FastQC (version 0.11.5)