FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8567545
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA79900.038.860336145
GTACTAA36500.018.7818681
GGGGGGG442400.018.53225145
GTATTAG41850.017.2429891
GTATTAA60700.016.0491891
GTGCTAG35950.015.3891881
GTCTTAA50700.014.9447741
CTATACT42800.014.8933154
GCCCTAG30450.014.8115571
TATACTG52900.014.6646245
TAACGTA11151.257184E-414.560525145
GTACTAG19850.014.5414441
GCCTTAG35050.014.4117951
GTCCTAG33800.014.4110331
TCTATAC41600.014.0218453
GCTCTAG40500.013.8087621
GTATTAT68250.013.7451261
GTATATA84500.013.66379451
GTTCTAA52700.013.4647461
GTACTAT34600.013.38262751
Produced by FastQC (version 0.11.5)