FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8567545
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA77800.026.223343145
CCTGTCG27250.023.209341145
CGCCGTA19650.019.726892145
GTATTAA61750.018.8028221
GTATTAG41800.018.709831
GTACTAA33750.018.5379731
GTACTAG21200.017.3099841
GTTCTAG44750.016.4009691
GTGCTAG35250.016.3838081
GTCGCCG27800.016.145277145
GTCCTAA33700.016.066281
GTCTTAG36800.015.8572051
CTATACT40050.015.76528554
CGAGTGT58250.015.761016145
CCTATAC26250.015.3484893
GTATATA84400.015.3249381
GTATAGG28800.015.0398231
TCTATAC38200.014.9547923
GTTCTAA52650.014.6256211
GCCGTAA11351.3978315E-414.3800955145
Produced by FastQC (version 0.11.5)