FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9549754
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA62450.046.087532145
GGGGGGG598250.019.958403145
GTACTAG17400.018.8213521
GTCCTAG37900.016.4031331
GTCCTAA30400.015.8852371
GTGCTAG36200.015.0267671
GTTCTAG40650.014.8838051
GTACTAA29300.014.7766171
GTCTTAG34750.014.5356541
GTATTAG31350.014.5185871
GTATAGG24500.014.2732381
CGAATCG7900.014.0504279
TATGCCG24550.013.929138145
GTACATA35400.013.3279611
GTATATA52150.013.1982211
GTGCTAA38450.013.1369061
GGTCTAG28750.012.7424851
GTATTAT48200.012.6675821
CTACTAA64400.012.5838931
GTTCTAA43700.012.57482051
Produced by FastQC (version 0.11.5)