FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9549754
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA64000.025.677622145
GTACTAG16650.020.3652861
CCGTATA8405.0483656E-420.23852145
CCTGTCG24553.45608E-1117.311972145
GTCCTAG35150.017.237571
GTATTAG30550.016.012031
GTTCTAA42900.015.1601481
GTACTAA26050.014.5102891
CTATACT33900.013.9331994
GTCTTAA44800.013.8968041
GCGCTAG6153.5135654E-613.821427145
GTGCTAG35450.013.79881
GGTCTAA28700.012.9768061
GTATTAA47250.012.9409381
GTCCTAA32700.012.9193521
GTCTTAG33300.012.6865711
GTCCTAT31300.012.6092411
GTATAAG31150.012.49150851
CGCCGTA18150.012.488783145
TATACTG44100.012.4746465
Produced by FastQC (version 0.11.5)