FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9623051
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA69700.033.64025145
CCTGTCG30150.027.774527145
GTACTAG17700.018.2584531
TGACGCG12552.2615206E-517.793402145
GTCCTAG36050.015.6108071
GTCGCCG37850.015.486959145
GTCTTAG34650.015.4375111
GTATTAG31450.015.2365641
GTGCTAG39450.014.6891151
GTCCTAA31150.014.6678031
GGTCTAG27050.014.6251511
GACGATA62050.014.395308145
TTGCGAT11850.00557547714.133367145
GTATACG8051.8189894E-1213.1512491
GTACTAA28350.012.9717971
GTCGCTT21601.2036648E-512.922871145
GCCGTAT23800.012.901151145
GCTCTAG44350.012.8149171
CGCCGTA22150.012.601986145
GTATATG46650.012.4219851
Produced by FastQC (version 0.11.5)