FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9065360
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG28550.023.261135145
CTGTCTA58200.022.060774145
GTCCTAG33550.019.4162561
GTACTAG17550.018.221411
GTTCTAG39600.016.3003391
GCGCTAG5458.731149E-1116.2989671
GTGCTAG36500.016.0622981
GTACTAA25850.015.807161
GGTCTAA26950.015.6014491
GTTCTAA39950.015.1198921
GTCTTAG31200.014.8048951
GTATTAG28800.014.3936481
GTATTAT41650.014.0762021
TAGCACG11050.00100577414.023341145
GTACATA39150.013.9162231
GTGTTAG36750.013.6970451
GTATACG7807.2759576E-1213.6660581
GGTCTAG28700.013.618441
GTCCTAA31250.013.2651861
GCCCTAG36150.013.1053021
Produced by FastQC (version 0.11.5)