FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9172956
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA62500.022.474878145
CCTGTCG32400.022.354572145
CGGTACG5650.00531214419.423119145
CTAGCGC6200.018.1870943
GTCCTAA32100.017.0120851
GTACTAG18650.016.5500491
GTCCTAG36350.016.3294051
GTCTTAG35400.016.0969181
GTCTTAA40450.015.7014581
GCTCTAG45450.015.2801191
GTACTAA25800.015.1844461
GTATTAG29500.015.0908591
GTACTAT28050.014.8128941
GGTCTAG31100.014.69621
GTGCTAG36700.014.3945691
GTATTAA45950.014.338781
GTTCTAA43500.014.3276441
TCTAGAC31200.014.266193
CTAGACT29800.013.7415014
GGTCTAA28600.013.6978581
Produced by FastQC (version 0.11.5)