FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9101412
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA60950.023.023216145
GTCCTAG36350.021.0648651
GTACTAG18100.019.0205361
GTACTAA27950.017.8390141
CCTGTCG33100.017.610111145
CTATACT29750.016.9590114
GTATTAG28350.016.331081
GTGCTAG35250.015.3234171
GTCCTAA32200.014.9314671
GCCGTAT20350.014.852172145
GTCTTAG34450.014.6454611
GTTCTAA40950.014.495041
GGTCTAT26650.014.4773651
GGTCTAA26350.014.1916631
GTTCTAG44150.014.116661
GCTCTAG44200.014.100691
CGCCGTA18550.013.965732145
TCTATAC31050.013.9553
GTATTAA47950.013.8644541
GTATATA47800.013.5354271
Produced by FastQC (version 0.11.5)