FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8061153
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT9700.036.60692145
CGCCGTT14850.032.207447145
GTACTAG14900.028.7646581
GTGCTAG32200.022.785521
CCGTATA12050.021.048433145
GTTCTAG37150.020.9227331
TTATACC73700.019.567007145
GTCCTAG35650.019.3578681
CGACGAA22600.018.277012145
GTATTAG30700.017.983271
TAGATCG11400.017.798851145
GTACATA38500.017.73621
GTACTAA25350.017.4801581
GACGATA26000.017.280533145
GGTCTAG25250.016.9739951
GTACAGA61450.016.9047071
GTATTAA43100.016.8545111
GTCTTAG31900.016.8513411
GTACAGG58700.016.8303951
GTATATG41700.016.5493531
Produced by FastQC (version 0.11.5)