FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7903908
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16100.025.6729811
GTACTAA25100.019.6454751
CGTCTTG39600.019.406137145
GTTCTAG33800.019.0941621
GTGCTAG30150.018.7600771
GTATTAG24750.018.4583421
GTATATA45750.018.2277931
GTATTAA40100.018.0835191
GTATATG40400.017.7697431
GTACAGA57850.017.4236351
CTATACT30450.017.3833184
GTCTTAG31050.017.2821371
GTCCTAG31600.016.981341
CTGTACT61400.016.8874764
GCTCTAG34450.016.6289651
GTACAGG56700.016.6259941
GTGCATA30600.016.3514021
TCTGTAC58100.016.3490413
CCCCTAG35650.016.0692231
CTATACG6357.530616E-1015.9864284
Produced by FastQC (version 0.11.5)