FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7903908
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT13500.034.905495145
CGCCGTT18250.023.834309145
GTCCTAG31400.022.8803371
GACGATA34850.022.258465145
GTGCTAG31500.021.6557941
GTACTAA25100.020.8168771
GGTCTAG23850.020.3865281
CCGTATA17600.019.359734145
CGACGAA23350.018.628529145
GTACTAG16850.018.0886521
GTATTAG25750.018.0368041
GTCTTAG28700.017.4471321
GTATATA48600.017.3212221
GCTCTAG38150.017.3102651
GTATAGG22800.017.187621
GTATTAA41050.017.1480751
TAGATCG16100.017.110863145
GCCGACC31750.016.668377145
CTAGACT28850.016.585514
GTTCTAG35450.016.5815681
Produced by FastQC (version 0.11.5)