FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8043807
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27000.020.1428181
GTACTAG16250.019.63461
CGTCTTA18150.019.172525145
GTTCTAG39950.019.0587881
GTGCTAG34100.018.7133271
GTCTTAG33250.017.66511
TAGACAG50850.017.5401845
GTACAGG60900.017.5034141
GCTCTAG38000.017.1744021
TCCTATG96100.016.973597145
GTATAGG22750.016.8934061
GTACTAA24800.016.6665571
CCTATCG26250.016.570541145
GTCCTAG32950.016.5054951
GTACTGG57150.016.2411381
CTATACT30850.016.2186224
GTACATA37650.016.1784551
GTCCTAC30700.016.0617641
AGACGGC88400.015.99179145
GTCCTAA28300.015.8864911
Produced by FastQC (version 0.11.5)