FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7949900
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT15200.026.222887145
CCGACGC99050.024.949465145
CCGTATA13050.024.434504145
CGTGTAT21350.020.366423145
GTGCTAG31800.020.3337881
TTACGTT23900.020.012781145
GACGACA33700.019.784237145
GTTCTAG39650.019.0566081
GTATAGG22300.018.8963781
GCCGACC32300.018.622454145
GTCCTAG31950.018.4191511
GTACTAA25700.018.3753531
CTAGCGT5651.4370016E-1017.9623814
GTCCTAA30050.017.891331
TGTACCG13850.017.7918285
GCGCTAG4506.159462E-817.75971
GTATTAG28000.017.6443771
GCCTTAG29650.017.6426261
GGTCTAA25950.017.0784281
GTACTAG15750.017.0677621
Produced by FastQC (version 0.11.5)