FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7809512
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15700.023.555491
GTATTAG26300.022.6089151
GTCCTAG34450.021.2595371
GGTCTAG25500.020.1901631
GTCTTAG31500.019.3370571
GTGCTAG31900.018.8672681
GTTCTAG37750.017.6722761
GTATAAG27700.017.0159111
CTGTACT63300.016.9538574
GTATTAA45850.016.922561
CGTCTTA27000.016.916565145
GTACTAT25000.016.5331841
GATCTAG27250.016.4985891
CGTCTTG39050.016.33793145
GTGCTAA33250.016.1384451
GCGCTAG5854.3819455E-916.1142121
GGTCTAA25750.016.0516341
GGGGGGG2785900.015.879692145
GTGCAGG110150.015.7996841
TGCCGTG49150.015.783222145
Produced by FastQC (version 0.11.5)