FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7699918
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16150.023.3441371
GTCCTAG33250.021.5869141
GTGCTAG27300.020.4491441
GTACTAA25850.017.6696131
CTCCGAC37700.017.500137145
GTATAAG27250.017.2939381
GTATAGG20850.017.0387231
GTTCTAG39750.016.2330421
GTATTAG26950.016.1413381
GGGGGGG2748250.015.923348145
GTCCTAA26950.015.8723151
GTACAGG57650.015.845951
GTTCTAA38000.015.8358551
GCTCTAA37100.015.829171
GGATTAG29950.015.7348851
GTATTAA40250.015.6711321
TCTATAC28700.015.662553
GCCCTAG33050.015.575211
CTAGACT30300.015.553334
TCTAGGC39000.015.4300093
Produced by FastQC (version 0.11.5)