FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7699918
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA20150.025.90363145
TTACGTT30550.023.017796145
GTCCTAG30900.022.538861
CGCCGTT18550.021.494234145
GTACTAG16100.020.7277011
GTATTAG26000.020.0899281
GTATAGG22250.019.8892781
GCCGACC36450.019.68978145
GTTCTAG33900.019.4742571
CCTAGAC28250.017.9654943
GTACTAA25050.017.9557321
GTCTTAG28900.017.5719261
CGTGTAT30300.017.465595145
GCCCTAG28650.017.2188221
TCTAGAC27700.017.0134813
GTCCTAA27300.017.0073451
TAGACAG49650.016.7921835
GGTCTAG23850.016.7299041
ATAGTAC24800.016.6641373
GTCTTAA38300.016.4793341
Produced by FastQC (version 0.11.5)