FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8107974
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA12650.026.925135145
CGCCGTT14300.024.325161145
GTACTAG15050.023.6545681
GTATTAG28350.021.783181
GTGCTAG35650.019.5644281
TTATACC63250.019.13403145
GTCCTAG33050.019.125081
GTTCTAA38750.018.7492431
GTTCTAG37750.018.4760741
GTATATA48000.017.2551611
GTATTAA38650.017.1059551
GTCTTAG28400.016.6283991
CTAGTAC16700.016.5046463
GTCTTAC40350.016.2052021
GGTCTAG25800.016.0513131
GTATAAG30100.015.9306281
CTGCCGC163000.015.916441145
GCCCTAG32250.015.76971151
GTACTAA25800.015.488111
GTACAGG62950.015.4655181
Produced by FastQC (version 0.11.5)