FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8054524
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16500.025.9289281
GTCCTAG29800.022.3865991
GTACTAA25750.019.7123031
GTGCTAG33200.019.0019281
TCTAGAC28650.018.4752563
GTTCTAG35000.018.2318671
GTATTAG27450.017.6990091
GCCCTAG30750.016.978681
GTATATA46750.016.90681
GTCTAGA29300.016.334011
GACGTAT25350.016.301786145
GTATTAA41450.016.2695271
TCTATAC27200.016.261223
CTAGACT28950.016.0296364
CGTCTTA29550.015.947558145
GTATAGA26400.015.930911
GTACAGA61000.015.9291091
CGTCTTG37800.015.919325145
GTACATA34950.015.768231
GGGGGGG3153000.015.64741145
Produced by FastQC (version 0.11.5)