FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7993844
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16850.021.9440571
GTCCTAG33700.021.2986431
GACGTAT24950.018.888979145
TACGCGA3602.6195848E-618.1262682
GTATTAA39000.017.2888051
GTATATA43600.017.1276341
GTATTAG27350.016.9656031
GTGCTAG30850.016.920921
GTCCTAA30150.016.832841
CGTCTTG45050.016.738026145
GTACTAA25950.016.483951
GCTCTAG35550.016.3153741
GTACTAT25450.016.2380451
CTATACT29250.016.112244
GTATATG40950.016.1114331
GTCTTAG33400.015.8461241
GTGTAGG43050.015.8307451
GCGCTAA5052.347333E-715.7923951
TGCCGTG53500.015.720628145
GGGGGGG3206100.015.35078145
Produced by FastQC (version 0.11.5)