FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7993844
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA17750.022.87128145
GTCCTAG31800.021.9028681
GTATTAG27650.020.99191
CGCCGTT15900.020.973047145
GTACTAG17000.020.9124071
GTTCTAA38600.018.4202561
TAGATCG18250.017.875183145
CGAATCG5751.8371793E-1017.6518789
GTACTAA24750.017.2955231
GTTCTAG35050.017.180941
GGTCTAG27900.017.1631341
CTAGACT26700.016.835664
GTATAGG23100.016.6464161
GCCCTAG32000.016.5512081
GTCTTAG31800.016.427151
GTGCTAA31800.015.9708411
GGTCTAA25900.015.9673171
GCTCTAG38700.015.9354681
GCGCTAG5955.4442353E-915.8519711
GTCCTAT27550.015.801071
Produced by FastQC (version 0.11.5)