FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6270432
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC87100.028.793793145
CCGTATA11850.024.467104145
CGCCGTT13050.021.106392145
GTATTAG18600.019.9225711
CTAGGCG5204.3655746E-1119.5251454
GTCCTAA23700.019.3143621
GTGCTAG23700.019.3143621
GTGTTAG24100.019.295281
TTACGTT14900.018.97227145
GTTCTAA30000.018.8912851
GTATAAG22000.018.8252321
GCTCTAG26250.018.545291
GCCGTAA10250.018.386135145
CGTGTAT15300.018.00251145
GTCCTAG23050.017.9676841
GCCGACC25250.017.798002145
GTATTAA31050.017.7844371
CCTCTAG37600.017.7782151
GTATATA31550.017.2722971
GTGTTAA27500.017.1738971
Produced by FastQC (version 0.11.5)